EPR spectra and antioxidant activity of gamma-irradiated papain

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅或者付费存取

详细

The structure and number of paramagnetic centers (PC) stabilized in papain γ-irradiated with a dose of 50 to 2300 kGy were studied using EPR spectroscopy. The radiation yield G≈6 PC/100 eV during radiolysis at 77 K is six times greater than the PC yield in the samples irradiated at 300 K. During radiolysis at 300 K, the maximum concentration of PC is achieved at doses of 200 kGy at a level of 8 × 1018 PC/g, whereas in papain radiolyzed at 77 K they accumulate up to 2300 kGy and reach 2.2 × 1020 PC/g. During papain radiolysis at 77 K, the cleavage of the peptide bond prevails over the cleavage of bonds in the molecular groups of amino acid residues, including sulfur-containing ones. As a result, C=0CHCH2R radicals are mainly recorded in the EPR spectra. In the multicomponent spectrum of papain irradiated at 300 K, a doublet with splittings of 1.77 mT is distinguished, attributed to the C=0CHNH radical formed by the abstraction of hydrogen from the glycine residue. Peroxide radicals formed during radiation oxidation at 300 K are not retained in the matrix of irradiated papain as stabilized radicals and, most likely, participate in secondary radiation-chemical processes with the formation of oxygen-containing products. A tendency for the antiradical and antioxidant activity of papain to increase with increasing radiation dose is noted as a result of radiation destruction of the peptide bond with the formation of amino acid fragments that are donors of a hydrogen atom.

全文:

受限制的访问

作者简介

S. Tokarev

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

I. Faingol’d

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

D. Poletaeva

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

A. Smolina

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

S. Demidov

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

A. Akimov

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

U. Allayarova

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

T. Raevskaya

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

S. Allayarov

Federal Research Center for Problems of Chemical Physics and Medicinal Chemistry of the RAS

编辑信件的主要联系方式.
Email: sadush@icp.ac.ru
俄罗斯联邦, Chernogolovka

参考

  1. Amri E., Mamboya F. // Am. J. Biochem. Biotechnol. 2012, V. 8. P. 99.
  2. Kamphuis I., Kalk K., Swarte M., Drenth J.J. // Mol. Biol. 1984. V. 179. P. 233.
  3. O´Neil M.J. The Merck index: an encyclopedia of chemicals, drugs, and biologicals, 13th Edition, Whitehouse Station, NJ: Merck, 2001.
  4. https://web.archive.org/web/20140715224627/http://www.biozym.de/datasheets/papain.php
  5. Menard R., Khouri H.E., Plouffe C., Dupras R., Ripoll D. // Biochemistry. 1990. V. 29. P. 6706.
  6. Tsuge H., Nishimura T., Tada Y., Asao T., Turk D. // Journal of Biochemicaland Biophysical Research Communications. 1999. V. 266. P. 411.
  7. Guo Z., Mcgill A., Yu L., Li J., Ramirez J., Wang P.G. // Bioorg. Med. Chem. Lett. 1996. V. 6. P. 573.
  8. Xian M., Chen X., Liu Z., Wang K., Wang P.G. // Journal of Biological Chemistry. 2000. V. 275. P. 20467.
  9. Varca G.H.C., Kadlubowski S., Wolszczak M., Lugão A.B., Rosiak J.M., Ulanski P. // Journal of Biolog. Macromolecules. 2016. V. 92. P. 654.
  10. Аллаяров С.Р., Руднева Т.Н., Демидов С.В., Аллаярова У.Ю., Чекалина С.Д. // Химия высоких энергий. 2024. Т. 58. № 5. С. 397.
  11. Аллаярова У.Ю., Демидов С.В., Блохина С.В., Раевская Т.А., Мищенко Д.В., Омельчук Ю.А., Аллаяров С.Р. // Химия высоких энергий. 2024. Т. 58. № 5. С. 404.
  12. Varca G.H.C., Ferraz C.C.F., Lopes P.S., Mathor M.B., Grasselli M., Lugão A.B. // Radiation Physics and Chemistry. 2014. V. 94. P. 181.
  13. Kedare S.B., Singh R.P. // J Food Sci Technol. 2011. V. 48. P. 412.
  14. Ohkawa N. Ohishi K., Yagi K. // Anal. Biochem. 1979. V. 95. P. 351.
  15. Шарпатый В.А. Радиационная химия биополимеров. М.: Энергоиздат, 1981. С.72.
  16. Sevilla M.D., D’Arcy J.B., Morehouse K.M. // J. Phys. Chem. 1979. V.83. P. 2893.
  17. Пшежецкий С.Я., Котов А.Г., Милинчук В.К., Рогинский В.А., Тупиков В.И. ЭПР свободных радикалов в радиационной химии ЭПР свободных радикалов в радиационной химии. М.: Химия, 1972. С. 309.
  18. Усатый А.Ф., Лазуркин Ю.С. Элементарные процессы химии высоких энергий. М.: Наука, 1965. С. 209.
  19. Garrison W.M., Jayko M.E., Rodgers A.J., Sokol H.A., Bennett-Corniea W. Washington, DC: The National Academies Press, 1968. P. 384.
  20. Кузина С.И., Аллаяров С.Р. // Химия высоких энергий. 2023. Т. 57. С. 384.
  21. Snipes W., Horan P.K. // Radiat. Res. 1967. V. 30. P. 307.
  22. Henriksen T. Electron spin resonance and effects of radiation on biological systems. Washington, DC: The National Academies Press, 1966. Р. 81.
  23. Каюшин Л.П., Львов К.М., Пулатова М.К. Исследование парамагнитных центров облученных белков, М.: Наука, 1970. С. 174.
  24. Милинчук В.К., Клиншпонт Э.Р., Пшежецкий С.Я. Макрорадикалы. М.: Химия, 1980. 264 с.

补充文件

附件文件
动作
1. JATS XML
2. Fig. 1. Dependence of the change in the concentration of PC on the dose of γ-irradiation at 77 K (a) and on the heating temperature (b) of papain irradiated at 77 K with a dose of 2300 kGy of the sample.

下载 (409KB)
3. Fig. 2. EPR spectrum of papain γ-irradiated at 77 K. Irradiation dose (kGy): 50 (a), 200 (b), 700 (c), 1700 (d), 2300 (e–i). Registration temperature (K): 77 (a–f), 223 (g), 273 (h), 300 (i). Samples (g–i) were registered during the heating of papain irradiated at 77 K to the indicated temperatures.

下载 (909KB)
4. Fig. 3. Kinetic curves of the accumulation of paramagnetic centers in papain during radiolysis at 300 K in vacuum (a) and in air (b).

下载 (358KB)
5. Fig. 4. EPR spectrum of papain γ-irradiated at 300 K in vacuum (solid lines) and in air (dashed lines). Irradiation dose (kGy): 50 (a), 200 (b), 700 (c), 1700 (d), 2300 (d). Registration temperature 300 K.

下载 (613KB)
6. Fig. 5. EPR spectra of papain γ-irradiated in air at 300 K. Irradiation dose (kGy): 50 (a), 200 (b), 3500 (c). Registration temperature (K): 77 (solid lines), 300 (dashed lines).

下载 (536KB)
7. Fig. 6. Interception of DPPH radical by irradiated papain, expressed as a percentage. Doses of γ-irradiation (kGy): 0 (a), 250 (b), 500 (c), 1550 (d), 3200 (d).

下载 (440KB)
8. Fig. 7. Inhibition of malondialdehyde formation as a lipid peroxidation indicator in mouse brain homogenate under the action of irradiated papain. Doses of γ-irradiation (kGy): 0 (a), 250 (b), 500 (c), 1550 (d), 3200 (d).

下载 (434KB)
9. Formula 1

下载 (30KB)
10. Formula 2

下载 (10KB)
11. Formula 3

下载 (11KB)
12. Formula 4

下载 (10KB)
13. Formula 5

下载 (3KB)
14. Formula 6

下载 (5KB)
15. Formula 7

下载 (5KB)

版权所有 © Russian Academy of Sciences, 2025