Optimization of biosynthesis of butyric acid from glucose through the inverted fatty acid β-oxidation pathway by recombinant Escherichia coli strains

封面

如何引用文章

全文:

开放存取 开放存取
受限制的访问 ##reader.subscriptionAccessGranted##
受限制的访问 订阅存取

详细

The biosynthesis of butyric acid from glucose though the inverted fatty acid β-oxidation by recombinant Escherichia coli strains was optimized. The increased yield of the target compound was achieved resulting from the plasmid expression of atoB, fadB and fadE/fabI genes in the core strain MG∆4 PL-tesB ΔyciA (MG1655 ∆ackA-pta, ∆poxB, ∆ldhA, ∆adhE, PL-SDj10-tesB, ∆yciA). The positive effect of enforced ATP hydrolysis on microaerobic conversion of carbohydrate substrate to the final product by the recombinants was demonstrated. Activation of the futile cycle of pyruvate-phosphoenolpyruvate-pyruvate, due to the increased expression of the ppsA gene, ensured a marked increase in glucose consumption by the recombinants and led to an increase in the molar yield of butyric acid up to 39.5%. When the components of the H+-ATP synthase complex were uncoupled resulting from the deletion of atpFH genes, the molar yield of butyric acid from glucose demonstrated by the strain forming butyryl-CoA by the action of enoyl-ACP reductase FabI reached 46%.

全文:

受限制的访问

作者简介

A. Gulevich

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: andrey.gulevich@gmail.com
俄罗斯联邦, Moscow, 117312

A. Skorokhodova

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: andrey.gulevich@gmail.com
俄罗斯联邦, Moscow, 117312

V. Debabov

Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences

Email: andrey.gulevich@gmail.com
俄罗斯联邦, Moscow, 117312

参考

  1. Dwidar M., Park J.Y., Mitchell R.J., Sang B.I. // Sci. World. J. 2012. V. 2012. 471417. https://doi.org/10.1100/2012/471417
  2. Sjoblom M., Risberg P., Filippova A., Ohrman O.G.W., Rova U., Christakopoulas P. // CemCatChem. 2017. V. 9. P. 4529–4537.
  3. Dürre P. // Biotechnol. J. 2007. V. 2. № 12. P. 1525–1534.
  4. Jha A.K., Li J., Yuan Y., Baral N., Ai B. // Int. J. Agric. Biol. 2014. V. 16. P. 1019–1024.
  5. Zigová J., Šturdı́k E. // J. Ind. Microbiol. Biotechnol. 2000. V. 24. P. 153–160.
  6. Luo H., Yang R., Zhao Y., Wang Z., Liu Z., Huang M., Zeng Q. // Bioresour. Technol. 2018. V. 253. P. 343–354.
  7. Volker A.R., Gogerty D.S., Bartholomay C., Hennen-Bierwagen T., Zhu H., Bobik T.A. // Microbiology. 2014. V. 160. P. 1513–1522.
  8. Saini M., Wang Z.W., Chiang C.J., Chao Y.P. // J. Agric. Food. Chem. 2014. V. 62. № 19. P. 4342–4348.
  9. Kataoka N., Vangnai A.S., Pongtharangkul T., Yakushi T., Matsushita K. // J. Biosci. Bioeng. 2017. V. 123. № 5. P. 562–568.
  10. Wang L., Chauliac D., Moritz B.E., Zhang G., Ingram L.O., Shanmugam K.T. // Biotechnol. Biofuels. 2019. V. 12. № 62. https://doi.org/10.1186/s13068-019-1408-9
  11. Серегина Т.А., Шакулов Р.С., Дебабов В.Г., Миронов А.С. // Биотехнология. 2009. № 6. С. 24–35.
  12. Гулевич А.Ю., Скороходова А.Ю., Дебабов В.Г. // Прикл. биохимия и микробиология. 2022. Т. 58. № 4. С. 330–337.
  13. Гулевич А.Ю., Скороходова А.Ю., Дебабов В.Г. // Прикл. биохимия и микробиология. 2021. Т. 57. № 2. С. 117–126.
  14. Sambrook J., Fritsch E., Maniatis T. // Molecular Cloning: a Laboratory Manual, 2nd Ed., N.Y.: Cold Spring Harbor Lab. Press, 1989. 1659 р.
  15. Скороходова А.Ю., Гулевич А.Ю., Дебабов В.Г. // Прикл. биохимия и микробиология. 2021. Т. 57. № 4. С. 342–352.
  16. Skorokhodova A.Y., Stasenko A.A., Krasilnikova N.V., Gulevich A.Y., Debabov V.G. // Fermentation. 2022. V. 8. № 12. 738. https://doi.org/10.3390/fermentation8120738
  17. Fujita Y., Matsuoka H., Hirooka K. // Mol. Microbiol. 2007. V. 66. № 4. P. 829–839.
  18. Vick J.E., Clomburg J.M., Blankschien M.D., Chou A., Kim S., Gonzalez R. // Appl. Environ. Microbiol. 2015. V. 81. № 54. P. 1406–1416.
  19. Koebmann B.J., Westerhoff H.V., Snoep J.L., Nilsson D., Jensen P.R. // J. Bacteriol. 2002. V. 184. № 14. P. 3909–3916.
  20. Vemuri G.N., Altman E., Sangurdekar D.P., Khodursky A.B., Eiteman M.A. // Appl. Environ. Microbiol. 2006. V. 72. № 5. P. 3653–3661.
  21. Hädicke O., Klamt S. // Biochem. Soc. Trans. 2015. V. 43. № 6. P. 1140–1145.
  22. Causey T.B., Shanmugam K.T., Yomano L.P., Ingram L.O. // Proc. Natl. Acad. Sci. U.S.A. 2004. V. 101. № 8. P. 2235–2240.
  23. Hädicke O., Bettenbrock K., Klamt S. // Biotechnol. Bioeng. 2015. V. 112. № 10. P. 2195–2199.

补充文件

附件文件
动作
1. JATS XML
下载

版权所有 © Russian Academy of Sciences, 2024