Protein Antioxidant Complex of Water Extract of Larvae of Black Beetles  Ulomoides dermestoides

N. A. Ushakova; Ушакова Н. А.; O. V. Tikhonova; Тихонова О. В.; A. V. Ambaryan; Амбарян А. В.; A. I. Bastrakov; Бастраков А. И.; A. E. Dontsov; Донцов А. Е.

doi:10.31857/S0555109923020162

Protein Antioxidant Complex of Water Extract of Larvae of Black Beetles Ulomoides dermestoides

作者: Ushakova N.A.¹, Tikhonova O.V.², Ambaryan A.V.¹, Bastrakov A.I.¹, Dontsov A.E.³
隶属关系:
1. Severtsov Institute of Ecology and Evolution, RAS
2. Institute of Biomedical Chemistry (IBMС)
3. Emanuel Institute of Biochemical Physics, RAS
期: 卷 59, 编号 3 (2023)
页面: 301-308
栏目: Articles
URL: https://permmedjournal.ru/0555-1099/article/view/674625
DOI: https://doi.org/10.31857/S0555109923020162
EDN: https://elibrary.ru/LVJJXJ
ID: 674625

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详细

An aqueous extract from the larvae of black beetles Ulomoides dermestoides grown under controlled conditions was obtained and its proteomic analysis based on one-dimensional electrophoretic separation of proteins, their trypsinolysis in a gel and subsequent chromatography-mass spectrometry was performed. Protein identification was performed using MaxQuant v.1.6.3.4 software. The Uniprot database was used to identify proteins by homology. Catalase activity was determined spectrophotometrically by hydrogen peroxide concentration decrease. Antioxidant activity was evaluated by luminol chemiluminescence quenching. The extract contained proteins that can determine the biological activity of the extract: universal regulators of cellular processes calmodulin (62%), cytochrome c-2 (13.5%), nucleoside diphosphate kinase (11.1%), the enzymatic antioxidants superoxide dismutase, catalase, glutathione S-transferase, peroxiredoxin, glutathione synthetase as well as thioredoxin, 70 and 60 kDa heat shock proteins, chitinase complex (13.4% in total). Catalase activity was 6.3 ± 1.1 µmol H₂O₂/min/mg protein; antioxidant activity of 1 mg protein per ml extract was equivalent to 1.36 ± 0.3 mM trolox. The prospects for practical use of the extract as a natural antioxidant complex were noted.

关键词

water extract, proteins, antioxidant, Ulomoides dermestoides larvae

参考

Suárez-Rivero J.M., Pastor-Maldonado C.J., Povea-Cabello S., Álvarez-Córdoba M., Villalón-García I., Munuera-Cabeza M. et al. // Antioxidants. 2021. V. 10. № 2. P. 236.https://doi.org/10.3390/antiox10020236
Pham-Huy L.A., He H., Pham-Huy C. // Int. J. Biomed. Sci. 2008. V. 4. № 2. P. 89–96.
Di Mattia C., Battista N., Sacchetti G., Serafini M. // Frontiers in nutrition. 2019. V. 6. P. 106. https://doi.org/10.3389/fnut.2019.00106
Mendoza D., Salgado M., Durant L. // Revista Cubana de Investigaciones Biomédicas. 2013. V. 32. P. 402–410.
Deloya-Brito G.G, Deloya C. // Acta Zoo Mex. 2014. V. 30. P. 655–661.
Mendoza-Meza D.L., España-Puccini P. // TIP Revista Especializada en Ciencias Químico-Biológicas. 2016. V. 19. № 2. P. 83–91.
Kovalzon V.M., Ambaryan A.V., Revishchin A.V., Pavlova G.V., Rybalkina E.Y., Bastrakov A.I., Ushakova N.A. // Systematic Reviews in Pharmacy. 2021. V. 12. № 10. P. 569–577.
Ushakova N.A., Brodsky E.S., Tikhonova O.V., Dontsov A.E., Bastrakov A.I. // Antioxidants. 2021. V. 10. P. 1055. https://doi.org/10.3390/antiox10071055
Laemmli U.K. // Nature. 1970 V. 227. № 5259. P. 680–685. . PMID: .https://doi.org/10.1038/227680a05432063
Tyanova S., Temu T., Cox J. // Nat. Protocols. 2016. V. 11. P. 2301–2319.
Sherman B.T., Hao M., Qiu J., Jiao X., Baseler M.W., Lane H.C., Imamichi T., Chang W. // Nucleic Acids Research. 2022. V. 50 (W1):W216–W221.https://doi.org/10.1093/nar/gkac194
Itzhaki R.F., Gill D.M. // Anal. Biochem. 1964. V. 9. P. 401–416.
Goth L. // Clinica Chimica Acta. 1991. V. 196. P. 143–15.
Stevens F.C. // Can. J. Biochem. Cell Biol. 1983. V. 61. № 8. P. 906–910. https://doi.org/10.1139/o83-115
Lledo P.M., Hjelmstad G.O., Mukherji S., Soderling T.R., Malenka R.C., Nicoll R.A. // Proc. Nat. Acad. Sci. USA. 1995. V. 92. № 24. P. 11175–11179.
Ващенко В.И., Хансон К.П., Шабанов П.Д. // Обз. клин. фарм. лек. тер. 2005. Т. 4. № 1. С. 27–37.
Липская Т.Ю., Воинова В.В. // Биохимия. 2012. Т. 77. С. 731–741.
Santoro M.G. // Biochem. Pharmacol. 2000. V. 59. № 1. P. 5563. https://doi.org/10.1016/S0006-2952(99)00299-329
Binder R.J. // J. Immunol. 2014. V. 193. № 12. P. 5765–5771. https://doi.org/10.4049/jimmunol.1401417
Marvin M., O’Rourke D., Kurihara T., Juliano C.E., Harrison K.L, Hutson L.D. // Dev. Dyn. 2008. V. 237. № 2. P. 454–463. https://doi.org/10.1002/dvdy.21414
Enzymes in Human and Animal Nutrition. Principles and Perspectives. Chapter 18 – Chitinases. //Ed. C. Simões Nunes, V. Kumar. N.Y.: Acad. Press, 2018. P. 361–378. ISBN 978-0-12-805419-2. https://doi.org/10.1016/B978-0-12-805419-2.00018-6