Perm Medical JournalPerm Medical Journal0136-14492687-1408Eco-Vector12105910.17816/pmj396125-132Research ArticleEffect of recombinant glycodelin on spleen structure in case of allogeneic bone marrow cells introductionTroynichYa. N.<p>Lecturer, Department of Histology, Embryology and Cytology</p>yananiktroynich@mail.ruLoginovaN. P.<p>MD, PhD, Professor, Head of the Department of Histology, Embryology and Cytology</p>yananiktroynich@mail.ruZamorinaS. A.<p>Doctor of Biological Sciences, leading researcher, Laboratory of Ecological Immunology of the Institute of Ecology and Genetics of Microorganisms UB RAS, Professor of the Department of Microbiology and Immunology</p>yananiktroynich@mail.ruRaevM. B.<p>Doctor of Biological Sciences, leading researcher of the Laboratory of Ecological Immunology of the Institute of Ecology and Genetics of Microorganisms of UB RAS, Professor of the Department of Microbiology and Immunology of Biological Faculty</p>yananiktroynich@mail.ruE.A. Vagner Perm State Medical UniversityPerm State National Research University060120233961251320601202306012023Copyright © 2023, Eco-Vector2023<p><strong>Objective.</strong> To study the effect of recombinant glycodelin (Mybiosource, Germany) on the morphofunctional state of the spleen in case of transplantation of the allogeneic red bone marrow cells to Wistar rats in dynamics of <em>in vivo</em> experiment. From the point of view of immunology, pregnancy is a physiologically conditioned state of the tolerance of mothers immune system to genetically foreign embryo. Glycodelin is a protein associated with pregnancy; it has an immunosuppressive effect and is perspective for medicine.</p>
<p><strong>Materials and methods.</strong> The morphological picture of the organ was assessed; the following immunohistochemical indicators were studied: monoclonal antibodies to 1) CD68 for identification of macrophages, membrane staining; 2) Ki-67 for cells divided with mitosis and being in different phases of cellular cycle; 3) determination of macrophage colony-stimulating factor (M-CSF).</p>
<p><strong>Results.</strong> When studying the histological slices of the spleen, it was shown that glycodelin against the background of allogeneic transplantation of the bone marrow contributes to the activation of immune system cells in the spleen, stimulates the proliferation of immune cells (Ki-67) and their differentiation that was manifested by an increase in the number of plasmacytes. By the end of the study, macrophage content is essentially reduced; eosinophil infiltration is verified that is an indirect positive sign of reaction to the transplant. Against the background of the bone marrow cells allotransplantation, there was observed an increase in M-CSF level in animals on the day 21<sup>st </sup>from the onset the experiment compared with the group of intact animals. Introduction of glycodelin against the background of BM cells allotransplantation caused the cancellation of this effect.</p>
<p><strong>Conclusions.</strong> Thus, the action of glycodelin qualitatively determined the function of the spleen in direction of the development of a tolerant immune response to allogenate and excluded the development of severe post-transplantation complications.</p>allogeneic transplantglycodelinred bone marrowspleenproliferationmacrophagesаллогенный трансплантатгликоделинкрасный костный мозгселезенкапролиферациямакрофаги[Afanas'ev B.V., Zubarovskaya L.S., Moiseev I.S. Allogeneic hematopoietic cell transplantation in children: present, problems, prospects. Rossiyskiy zhurnal detskoy gematologii i onkologii 2015; 2: 28–42 (in Russian).][Martin P.J., Rizzo J.D., Wingard J.R. et al. First- and second-line systemic treatment of acute graft-versus-host disease: recommendation of the American Society of Blood and Marrow Transplantation. Biol. Blood Marrow Transplant. 2012; 18 (8): 1150–1163.][Popova N.N., Savchenko V.G. Reconstitution of T-cell-mediated immunity in patients after allogeneic stem sell transplantation. Russian journal of hematology and transfusiology 2020; 65 (1): 24–38.][Dixit A., Balakrishnan B., Karande A.A., Immunomodulatory activity of glycodelin implications in allograft rejection. Clin. Exp. Immunol. 2018; 2: 213–223.][Cui J., Liu Y., Wang X. The Roles of glycodelin in cancer development and progression. Front. Immunol. 2017; 8: 1685.][Kämäräinen M., Halttunen M., Koistinen R., von Boguslawsky K., von Smitten K., Andersson L.C., Seppala M. Expression of glycodelin in human breast and breast cancer. Int. J. Cancer 1999; 83: 738–742.][Zamorina S.A., Troynich Y.N., Loginova N.P., Charushina Y.A., Shardina K.Yu., Timganova V.P. Pregnancy-associated proteins as a tool in the therapy of autoimmune diseases and alloimmune disorders (review). Lecture notes in networks and systems 2022; 342 LNNS: 385–393.][Reddy P., Ferrara J.L.M. Mouse models of graft-versus-host disease. StemBook eds. By The Stem Cell Research Community. 2009; 28.]