Perm Medical JournalPerm Medical JournalПермский медицинский журнал (сетевое издание "Perm medical journal")0136-14492687-1408Eco-Vector5958010.17816/pmj37613-24Original studiesОригинальные исследованияResearch ArticleFeatures of amniotic fluid microbiota in full-term pregnancyОсобенности микробиоты околоплодных вод при доношенной беременностиKaganovaM. A.КагановаМ. А.
Russian Federation

Candidate of Medical Sciences, Associate Professor, Department of Obstetrics and Gynecology

кандидат медицинских наук, доцент кафедры акушерства и гинекологии

mkaganova@yandex.ruSpiridonovaN. V.СпиридоноваН. В.
Russian Federation

MD, PhD, Professor, Head of Department of Obstetrics and Gynecology

доктор медицинских наук, профессор, заведующая кафедрой акушерства и гинекологии

mkaganova@yandex.ruMedvedchikova-ArdiyaL. K.Медведчикова-АрдияЛ. К.
Russian Federation

obstetrician-gynecologist

акушер-гинеколог

mkaganova@yandex.ruSamara State Medical UniversityСамарский государственный медицинский университетSamara Regional Clinical Hospital named after V.D. SeredavinСамарская областная клиническая больница имени В.Д. Середавина2812202037613242701202127012021Copyright ©; 2021, Kaganova M.A., Spiridonova N.V., Medvedchikova-Ardiya L.K.Copyright ©; 2021, Каганова М.А., Спиридонова Н.В., Медведчикова-Ардия Л.К.2021Kaganova M.A., Spiridonova N.V., Medvedchikova-Ardiya L.K.Каганова М.А., Спиридонова Н.В., Медведчикова-Ардия Л.К.http://creativecommons.org/licenses/by-sa/4.0https://permmedjournal.ru/PMJ/article/view/59580

Objective. To study the microbial landscape of amniotic fluid in physiological process of full-term pregnancy. Recently, after publication of a number of studies regarding human microbiota (The Human Microbiome Project – HMP), there occurred a change in paradigm on absolute sterility of fetal membranes and amniotic fluid in physiologically developing pregnancy.

Materials and methods. At the City Clinical Hospital № 1 named after N.I. Pirogov, during elective cesarean section of 19 pregnant women (at the terms of 37–41 weeks) with intact fetal membranes, an amniotic fluid of the following microorganisms was taken by means of PCR-PB: Lactobacillus spp., Enterobacteriaceae, Streptococcus spp., Staphylococcus spp., Gardnerella vaginalis / Prevotella bivia / Porphyromonas spp., Eubacterium spp., Sneathia spp. / Leptotrihia spp. / Fusobacterium spp., Megasphaera spp. / Veillonella spp. / Dialister spp., Lachnobacterium spp. / Clostridium spp., Mobiluncus spp. / Corynebacterium spp., Peptostreptococcus spp., Atopobium vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Mycoplasma henitalium.

Results. The general bacterial mass (GBM) of amniotic fluid in intact fetal membranes is 103,02 Ge/copies, in 47.4 % of cases the amniotic fluid is sterile. Microbiota is most often presented by Enterobacteriaceae spp. – 37 %, the share of the rest, identified bacteria is 28 %, the share of “unknown” is 35 %.

Conclusions. In case of physiologically developing pregnancy and intact fetal membranes, the general bacterial mass is low (GBM = 103,02 ± 345 Ge/ml). In the intact amniotic sac the most typical microorganisms living in amniotic fluid are Enterobacteriaceae spp. (37 %), the rest are presented in single instances. The presence of the representatives of anaerobic vaginal dysbiosis as well as lactobacilli is not typical for the intact fetal membranes.

Цель. Изучить микробный пейзаж околоплодных вод при физиологическом течении доношенной беременности. В последние несколько лет после публикации ряда исследований, связанных с изучением микробиома человека (The Human Microbiome Project – HMP), произошла смена парадигмы о безусловной стерильности плодных оболочек, плаценты и околоплодных вод при физиологически протекающей беременности.

Материалы и методы. У 19 беременных в сроке 37–41 неделя с интактными плодными оболочками во время элективного кесарева сечения проводился забор амниотической жидкости на ПЦР-РВ следующих микроорганизмов: Lactobacillus spp., Enterobacteriaceae, Streptococcus spp., Staphylococcus spp., Gardnerella vaginalis / Prevotella bivia / Porphyromonas spp., Eubacterium spp., Sneathia spp. / Leptotrihia spp. / Fusobacterium spp., Megasphaera spp. / Veillonella spp. / Dialister spp., Lachnobacterium spp. / Clostridium spp., Mobiluncus spp. / Corynebacterium spp., Peptostreptococcus spp., Atopobium vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Mycoplasma henitalium.

Результаты. При интактных плодных оболочках общая бактериальная масса (ОБМ) околоплодных вод составляет 103,02 Гэ/копий, в 47,4 % случаев околоплодные воды стерильны. Микробиота наиболее часто представлена семейством Enterobacteriaceae spp. – 37 %, на долю остальных идентифицированнных бактерий – приходится 28 %, на долю «неизвестных» – 35 %.

Выводы. При физиологически протекающей беременности и интактных плодных оболочках общая бактериальная масса околоплодных вод низкая (ОБМ = 103,02 ± 345 Гэ/мл). При целом плодном пузыре наиболее характерными видами микроорганизмов, обитающими в амниотической жидкости, является Enterobacteriaceae spp. (37 %), остальные представлены в единичных случаях. Наличие представителей анаэробного дисбиоза влагалища, а также лактобацилл нехарактерно при целых плодных оболочках.

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