Influence of Bioaugmentation of Bacillus subtilis, B. amyloliquefaciens, Pseudomonas aeruginosa on the Efficiency of Food Waste Composting

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Abstract

The use of inoculum containing autochthonous compost microorganisms Bacillus subtilis, B. amyloliquefaciens, Pseudomonas aeruginosa allowed to enhance biodegradation of food waste during composting. The survival of the introduced microorganisms was verified by classical microbiological and molecular biological methods. The introduction extended the high-temperature stage by 4 days, prevented acidification of the medium, increased organic matter degradation and moisture evaporation, resulting in a 51% reduction in waste weight in two weeks. Introduced P. aeruginosa appeared to play a key role in the initial stage and was not detected after increasing the temperature to 60C.

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About the authors

V. V. Mironov

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Author for correspondence.
Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

A. A. Shchelushkina

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

V. V. Ostrikova

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

A. A. Klyukina

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

A. V. Vanteeva

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

I. A. Moldon

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

V. G. Zhukov

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

I. B. Kotova

Moscow State University

Email: 7390530@gmail.com

биологический факультет

Russian Federation, Moscow, 119991

Yu. A. Nikolaev

Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences

Email: 7390530@gmail.com
Russian Federation, Moscow, 119071

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2. Fig. 1. Dynamics of changes in the composting conditions of the substrate with bioaugmentation (B) and the control substrate (K): a — substrate temperature (T), ambient temperature (Tn); b — pH; c-oxygen consumption (O2); d — loss of dry matter (mK, mB) and humidity (wK, wB) (the error bars represent the average value ± standard deviation).

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