Effect of silver nanoclusters on the copper resistance of Achromobacter insolitus LCu2

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Abstract. In this work, the resistance of Achromobacter insolitus LCu2 cells to copper (II) was reduced by adding 1 μM silver nanoclusters to the culture medium: the maximum tolerable concentration decreased by 4 times, the minimum inhibitory concentration – by 25 times. It is assumed that nanoclusters disrupt the functioning of the copper (II) efflux system through binding to the CusC protein, which leads to a partial loss of the ability of bacteria to export excess copper (II) cations from cells.

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G. Burygin

Saratov Scientific Centre of the Russian Academy of Sciences; Saratov State University; Saratov State University of Genetics, Biotechnology, and Engineering named after N.I. Vavilov

编辑信件的主要联系方式.
Email: burygingl@gmail.com

Institute of Biochemistry and Physiology of Plants and Microorganisms

俄罗斯联邦, Saratov, 410049; Saratov, 410012; Saratov, 410012

A. Astankova

Saratov Scientific Centre of the Russian Academy of Sciences; Saratov State University

Email: burygingl@gmail.com

Institute of Biochemistry and Physiology of Plants and Microorganisms

俄罗斯联邦, Saratov, 410049; Saratov, 410012

D. Chumakov

Saratov Scientific Centre of the Russian Academy of Sciences

Email: burygingl@gmail.com

Institute of Biochemistry and Physiology of Plants and Microorganisms

俄罗斯联邦, Saratov, 410049

Y. Kryuchkova

Saratov Scientific Centre of the Russian Academy of Sciences; Saratov State University of Genetics, Biotechnology, and Engineering named after N.I. Vavilov

Email: burygingl@gmail.com

Institute of Biochemistry and Physiology of Plants and Microorganisms

俄罗斯联邦, Saratov, 410049; Saratov, 410012

参考

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3. Fig. a – Effect of different concentrations of copper (II) cations in the medium (1) and combined action of 1 μM GSH-AgNCs with copper (II) cations (2) on the viability of the A. insolitus LCu2 strain culture; b – 3D model of the efflux pump – protein complex formed by the CusA trimer, CusB hexamer and CusC trimer of the A. insolitus LCu2 strain; c – horizontal projection of the 3D model of the CusBC protein complex forming a transport pore in the outer cell membrane (copper (II) cations are shown as balls).

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