LOCAL IMMUNE RESPONSE OF CERVICAL MUCUS NEUTROPLILES IN WOMEN WITH INFLAMMATORY DISEASES OF LOWER UROGENITAL PART UNDER THE EFFECT OF HEALTHY MEN'S DIFFERENT SPERM FRACTIONS

The possibility of normal functioning of female reproductive system is provided by immunological barriers and normal realization of immunoregulating mechanisms. Immune hemostasis is changed in case of inflammatory diseases of reproductive tract that can impair the normal reproductive process. Aim. To determine the mechanisms of local immune response of cervical mucus neutrophiles in women with inflammatory diseases of the lower part of urogenital tract (IDLPUT) under the effect of healthy men's sperm and fractions. Materials and methods. Cervical mucus of 20 women with IDLPUT (by the results of microscopic, bacteriological, serological, molecular-biological methods of study) in the first phase of menstrual cycle was placed into 1,0 ml of Hanks' solution, thoroughly suspended and studied. Seminal fluid of 20 practically healthy men was divided into components by centrifugation during 10 minutes at 3000 turns per minute. Thus, supernatant and sedimentary parts of ejaculate were produced. For further study, cervical mucus and Hanks' solution, cervical mucus and whole seminal fluid, cervical mucus and sperm sediment, cervical mucus and supernatant part of sperm were incubated for 30 minutes at 37ºC in the ratio of 1:1. After incubation, viability and functional activity of cervical secretion neutrophiles was assessed. Viability and functional activity values of cervical mucus neutrophile suspension in Hanks' solution were accepted as the control (group 1). Comparison groups included viability and functional activity values of cervical mucus neutrophiles under the effect of whole sperm (group 2), under the effect of sedimentary fraction of sperm (group 3), under the effect of supernatant fraction of sperm (group 4). Results. Under the effect of healthy men's sperm supernatant, phagocytic function of cervical mucus neutrophiles in women with IDLPUT is growing as compared to the effect of sediment when phagocytosis indices reliably decrease. When analyzing viability of cervical mucus cells (in women with IDLPUT) under the effect of different fractions of healthy men's sperm, there are observed changes in the ratio of cells during different phases of vital cycle. Supernatant part of sperm significantly lowered the number of bioplasts. Conclusions. Whole seminal fluid and its supernatant fraction decrease viability of cervical mucus neutrophiles; sedimentary fraction has inhibiting action on phagocytic function of cervical mucus neutrophiles, whereas supernatant fraction stimulates it.

Neutrophiles, cervical mucus, sperm, phagocytosis, viability