Cite item


Nowadays, immunological aspect of sterility is an important and priority direction to be studied. Aim. To study the effect of seminal fluid and cell elements of ejaculate received form healthy men on lysosomal, phagocytic activity of neutrophiles secreted from female peripheral blood as well as intracellular oxygen-dependent metabolism and ability of these cells to form extracellular nets. Materials and methods. The level of neutrophilic extracellular trap and functional activity of neutrophiles secreted from peripheral blood of 17 healthy women (aged 19-35) during the first phase of menstrual cycle under the effect of seminal fluid (SF) and ejaculate cell elements (ECE) of healthy men (aged 18-36) in vitro was assessed. Spermiologic analysis included WHO standard indices. Seminal fluid characterized by normozoospermia state was used in the study. Seminal fluid DNCase concentration was determined with the method of immunofermental analysis. Neutrophilic granulocyte functional activity indices to be studied were the following: lysosomal, phagocytic activity, intracellular oxygen-dependent metabolism of the studied cells. Simultaneously, the experiment using the drug “pyrogenal” as a neutrophile activator was performed. The same indices influenced by SF and ECE were estimated. Results. Decreased phagocytic activity of neutrophilic granulocytes was stated. The level of extracellular nets formed by neutrophiles was shown to fall under the effect of seminal fluid and to raise while interacting with ejaculate cell elements. The role of pyrogenal as an activator is proved by statistical results. Conclusions. Phagocytic activity of neutrophilic granulocytes is decreased under the effect of not only seminal fluid, but ejaculate cell elements, as well. Neutrophiles interacting with seminal fluid and cell elements of ejaculate are activated on the way of NETosis. Seminal fluid of healthy men possessing DNCase activity contributes to destruction of neutrophilic extracellular traps.

About the authors

A A Savelieva



  1. Бабаян А. А., Смольникова В. Ю., Николаева М. А., Степанова Е. О., Калинина Е. А. Влияние иммунорегуляторных свойств семенной плазмы на репродуктивную функцию женщин. Гинекология 2012; 4: 80-82.
  2. Виксман М. Е., Маянский А. Н. Способы оценки функциональной активности нейтрофилов человека по реакции восстановления нитросинего тетразолия: метод. рекомендации. Казань: Казанский НИИЭМ 1979; 11.
  3. Долгушин И. И., Андреева Ю. С. Способ обнаружения нейтрофильных ловушек: пат. № 2384844 Рос. Федерации (Российская Федерация). Заявл. 01.04.2008; опубл. 20.03.2010. Бюл. № 8; 7.
  4. Troedsson M. H. T., Desvousges A., Alghamdi A. S., Dahms B., Dow C. A., Hayna J., Valesco R., Collahan P. T., Macpherson M. L., Pozor M., Buhi W. C. Components in seminal plasma regulating sperm transport and elimination. Animal Reproduction Science 2005; 89 (1): 171-186.
  5. Abdorrahman S. Alghamdi A.S., Bethany J., Funnell, Scott L Bird, G. Cliff Lamb, Aaron K. Rendahl, Patrick C. Taube, Douglas N. Foster Comparative studies on bull and stallion seminal DNase activity and interaction with semen extender and spermatozoa. Animal reproduction science 09/2010; 121 (3-4): 249-258.
  6. Abdorrahman S., Alghamdi A. S., Bethany J. Lovaas, Scott L. Bird, G. Cliff Lamb, Aaron K. Rendahl, Patrick C. Taube, Douglas N. Foster Species-specific interaction of seminal plasma on sperm-neutrophil binding. Animal Reproduction Science 2009; 114 (4): 331-344.
  7. Fuchs T. A., Abed U., Goosmann C. Novel cells death program leads to neutrophil extracelllular traps. J. Cell. Biol. 2007; 176 (2): 231-241.

Copyright (c) 2014 Savelieva A.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies