Vol 32, No 3 (2015)

Articles
COMPLEX ASSESSMENT OF RIGHT VENTRICULAR FUNCTION IN PATIENTS WITH MULTIVASCULAR CORONARY artery LESIONS
Osetrova O.A., Orekhova E.N.
Abstract
Aim. To carry out complex assessment of the right ventricular function in patients with multivascular coronary lesions. Materials and methods. Sixty three patients aged 58,5 ± 9 years including 46 men (73 %) with multivascular coronary lesions were examined. In addition to general clinical protocol of the study, the results of selective coronarography with determination of SYNTAX Score, MRI with contrast gadolinium strengthening and EchoCG with studying of tissue myocardial dopplerography and 2D-speckle tracking echocardiography were compared. Results. The signs of mechanical right ventricular myocardial dysfunction with preserved systolic function and standard right ventricular volumes by MRI data were detected. In particular, segmental deformation and deformation rate indices were changed. Manifestation of inhibited systolic dysfunction of the right ventricle depended on complexity and severity of coronary lesions: moderate invert correlation of SYNTAX Score and that of right ventricular ejection fraction by MRI data ( R s = -0,6) was observed. The higher is SYNTAX Score index, the more expressed is myocardial dysfunction (Tei index, R s = -0,4) and the lower is deformation at the level of tricuspid valve fibrous ring ( R s = -0,5). Conclusions. Tissue myocardial dopplerography and 2D-speckle tracking echocardiography included into the protocol of examining patients with multivascular coronary lesions for the purpose of assessment of the right ventricular function permits to confirm its involvement into the process of ischemic heart remodeling.
Perm Medical Journal. 2015;32(3):6-13
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APPLICATION OF BOWEL COMPRESSION STAPLER IN SURGERY OF LEFT HALF COLONIC CANCER
Vlasov A.A., Vazhenin A.V., Plotnikov V.V., Vlasov A.V., Fedorov S.V.
Abstract
Aim. To improve the results of surgical treatment in patients with cancer of the left half of the colon using a new technique for forming circular compression anastomosis. Materials and methods. The perspective clinical study was based on the results of surgical treatment of patients with cancer of the left half of the colon from 2009 to 2015. Patients were divided into 2 groups according to the techniques used for anastomosis formation. Group 1 included patients with hand-sewn double layer suture ( n = 64), group 2 - with compression anastomosis ( n = 63). Results. While fulfilling comparative assessment of the early postoperative complications, statistically significant decrease in anastomosis failure ( р = 0,043), total number of complications ( р = 0,002) and lethality ( р = 0,043) was revealed in the group with compression stapler used as compared to the hand suture. Conclusions. Application of new compression anastomosis technique is an effective and reliable method for restoration of continuity of the colon in oncological patients.
Perm Medical Journal. 2015;32(3):14-21
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MANAGEMENT TACTICS FOR SUFFERERS WITH POLYTRAUMA IN ACUTE PERIOD OF TRAUMATIC DISEASE
Shabanova A.Y.
Abstract
Aim. To develop the management tactics used in the struggle for life during the acute period of traumatic disease in sufferers with multiple long bone fractures, which are characterized by shock, external and internal hemorrhage, fat embolism. Materials and methods. The study involved 156 patients with polytrauma over the period of 2009-2013. Three groups of patients, who experienced different ways of rendering intensive therapy at the prehospital and hospital stages, were formed. Results. Sufferers with multiple and combined injuries of musculoskeletal system (MSS) who underwent surgical intervention in the acute traumatic period improved their health faster than those who underwent surgery in the late traumatic period, that was confirmed by the basic hemodynamic indices ( p < 0,05). Surgical techniques during the first day after the injury, which were applied for all types injuries, both abdominal and orthopedic (ISS < 25 scores), promoted stabilization of patients’ health status and were antishock measures. With introduction of new approaches for the treatment of traumatic disease in the acute period, the terms of staying at the resuscitation and intensive therapy units reduced from 2,98 to 1,86 days and at the profile traumatological unit from 13,12 to 11,78 days. Conclusions. Introduction of a new antishock algorithm permitted to perform surgeries in sufferers with polytrauma not only by vital indices, but to use early surgical tactics for management of patients with multiple and combined MSS injuries.
Perm Medical Journal. 2015;32(3):22-28
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PECULIARITIES OF ENTERAL INSUFFICIENCY COURSE AGAINST THE BACKGROUND OF ACUTE ABDOMINAL PATHOLOGY
Takha K.D., Allniazi A.E., Amakhanov A.K., Inyutin A.S.
Abstract
Aim. To assess the peculiarities of enteral insufficiency syndrome at the example of patients with disseminated peritonitis. Materials and methods. The work was based on examination of 98 patients with purulent peritonitis. The severity of enteral insufficiency syndrome (EIS) taking into account peritonitis staging was estimated. Procalcitonin level as a marker of septic process was studied. Results. Among patients with reactive stage of peritonitis, only degrees I and II EIS were found. In patients with the toxic stage of disseminated peritonitis there predominated degree II EIS with the rate of 60,8 % (31 persons); degree III EIS was detected in 14 cases (27,4 %). At the terminal stage of peritonitis, degree III EIS was detected in 80 % of patients and degree II EIS - in 20 %. Blood procalcitonin level exceeded the norm that indicated bacterial nature of intoxication. Conclusions. There is a direct correlation between the enteral insufficiency syndrome and the peritonitis severity; procalcitonin level is closely associated with peritonitis severity and enteral insufficiency.
Perm Medical Journal. 2015;32(3):29-33
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CEREBROVASCULAR DISTURBANCE PREDICTORS IN PATIENTS AFTER CORONARY ARTERY BYPASS GRAFT SURGERY
Sukhanov S.G., Marchenko A.V., Myalyuk P.A., Arutyunyan V.B., Chragyan V.A., Vronsky A.S.
Abstract
Aim. To detect the perioperative insult predictors in patients with isolated coronary artery bypass grafting. Materials and methods. At Perm Federal Center of Cardiovascular Surgery, 2823 coronary artery bypass graft surgeries were performed over the period from January 2013 to December 2014. Results. The total hospital lethality among patients with isolated coronary artery bypass grafting was 1,2 % (36). Perioperative acute stroke (AS) was revealed in 32 (1,1 %) patients. To carry out the analysis, we divided patients into 2 groups: group A - 32 patients with AS diagnosed in the postoperative period, and group B - 2791 patients without severe cerebrovascular disorders. The analysis showed that in group A, there were significantly more female patients - 13 (40,6 %) versus 543 (19,5 %) in group B ( p < 0,01) as well as patients over 60: 21 (65,6 %) and 1251 (44,8 %), respectively ( p < 0,05); over 70: 9 (2,1 %) and 348 (12,5 %) patients, respectively ( p < 0,05). In group B, the number of patients with rhythm disturbance by atrial filbrillation type was 244 (8,7 %), in group A - 7 (21,9 %), that is reliably higher ( p < 0,01). In AS group, diabetes mellitus was found in 12 (37,5 %) patients, in group without AS - in 212 (7,6 %) ( p < 0,01). Significant differences were obtained by the quantity of patients with brachiocephalic artery atherosclerosis - 17 (53,1 %) in group A and 624 (22,4 %) in group B ( p < 0,01), patients with low extremity artery atherosclerosis - 16 (50,0 %) and 715 (25,6 %), respectively ( p < 0,01). Conclusions. The most significant predictors influencing the risk for occurring perioperative insult are concomitant brachiocephalic artery atherosclerosis, low extremity artery atherosclerosis, rhythm disturbance by atrial filbrillation type, diabetes mellitus, middle age.
Perm Medical Journal. 2015;32(3):34-40
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CLINICOLABORATORY PECULIARITIES OF INFECTIOUS MONONUCLEOSIS IN PRESCHOOL CHILDREN
Postanogova N.O.
Abstract
Aim. To study the clinical and laboratory peculiarities of infectious mononucleosis (IM) in preschool children depending on etiological factor. Materials and methods. Clinicolaboratory peculiarities of IM depending on etiological factor were analyzed in 60 patients aged 1-6 years (20 patients with Epstein-Barr virus-associated IM (EBV-IM), 20 - cytomegalovirus (CMVI) IM and 20 - mixed etiology mononucleosis (EBV+CMVI). Results. It was established that EBV-IM- caused intoxication occurred reliably more often than CMVI-IM (EBV-IM 18 patients, CMVI-IM 8 patients, p = 0,02). In case of mixed IM, splenomegalia occurred more often than in IM caused by one etiological factor (mixed IM - 18 children, EBV-IM - 13, CMVI-IM - 13, p = 0,05). In case of EBV-IM and mixed IM, all changes in blood were more marked than in CMVI-IM. Conclusions. Against the background of therapy, fiver and intoxication were the first to disappear. The most long preserved symptom was stuffiness in nose. Lymphomonocytosis, transaminasemia, atypical blood mononuclear leukocytes were more often observed in EBV-IM and mixed etiology IM. During one year after discharge from the hospital, all children more often fell ill with respiratory diseases, especially in case of CMVI-IM.
Perm Medical Journal. 2015;32(3):41-45
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ROLE OF RESPIRATORY TRACT INFECTION IN FORMATION OF INFANTILE ATOPIC PHENOTYPE
Bukina E.V.
Abstract
Aim. To study the formation of atopic phenotype in children who had respiratory infection in the newborn period. Materials and methods. Retrospective study of 36 children who had respiratory infection in the neonatal period with subsequent examination at the age of 5-6 years was carried out on the basis of Perm Children’s City Clinical Hospital № 13. Results. Children with congenital pneumonia in 94,4 % of cases were found to suffer from atopic dermatitis, that is reliably more often than children with acute respiratory infection (50,0 %) in the neonatal period ( p < 0,01). All children with newborn respiratory infection form later the group of children who frequently fall ill. However, in the group of children with congenital pneumonia, complications of acute respiratory diseases occur more often, in particular, bronchoobstructive syndrome (44,4 % vs. 5,6 %, p < 0,05). No reliable differences between the groups were detected while studying respiratory resistance (hyperreactivity of bronchi was observed in 33,3 and 45,5 % of cases, respectively). In children who suffered from congenital pneumonia, IgE level is raised significantly more often ( p < 0,05). Conclusions. Children with congenital pneumonia, later on have high susceptibility to respiratory infections with development of complications that is an unfavorable factor for development of allergic diseases. Presence of atopic dermatitis, bronchial hyperreactivity and elevated sensitizing level contributes to formation of atopic phenotype as a risk factor for development of bronchial asthma.
Perm Medical Journal. 2015;32(3):46-50
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METHOD FOR BIOLOGICAL AGE DETERMINATION IN PREGNANT WOMEN
Statnykh N.V., Belozerova L.M.
Abstract
Aim. To develop the method for determination of biological age by blood, abdominal circumference and uterine fundus height indices in women of I, II and III trimester of pregnancy. Materials and methods. Two hundred pregnant women aged 20 to 39 years with physiologically developing pregnancy were examined. The inclusion criteria were the following: monocyesis, chronological age 20-39 years, uncomplicated course of pregnancy. The exclusion criterion was use of pharmaceutical preparations in the first trimester of pregnancy. Women were examined in each trimester of pregnancy. Results. For each trimester the following formulas for BA and PBA calculation were received. As a result of calculations it was stated that practically all the examined women have physiological tempo of aging in the course of pregnancy. Conclusions. We consider that the method for biological age determination by laboratory indices for pregnant women in I, II and III trimester is a simple, available and objective method aimed at estimation of the tempo of age changes in pregnancy.
Perm Medical Journal. 2015;32(3):51-55
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PECULIARITIES OF COGNITIVE STATUS IN YOUNG WOMEN WITH MILD AND MODERATELY SEVERE IRON-DEFICIENCY ANEMIA
Shavrina E.S.
Abstract
Aim. To give the comparative characteristic of cognitive status in women depending on presence of iron-deficiency anemia, to study the possibilities of restoring the available cognitive deficiency against the background of anemia correction with iron preparations. Materials and methods. The study included 40 women aged 18 to 44 years, who were diagnosed mild and moderately severe iron-deficiency anemia. Complex clinicolaboratory and neuropsychical examination was carried out using MoCa test, Gotland, Spilberger-Hanin scales, asthenia estimation scale (MFI-20) before anemia therapy, 12 and 24 weeks after it. Comparison was fulfilled with the control group of nonanemic women. Results. When analyzing cognitive functions before anemic therapy in the group of mild and moderately severe iron-deficiency anemic patients aged 18 to 44 years, we detected mild cognitive disorders, predominantly attention, speech and memory functions. Anemia correction, normalization of hemoglobin, iron and blood serum ferritin against the background of anemia therapy with iron preparation were associated with reduced manifestation of cognitive disorders. Conclusions. The study results indicated that ferrotherapy reliably restores the available cognitive deficiency in young patients diagnosed mild and moderately severe iron-deficiency anemia. However, despite restoration of hematological indices, correction of cognitive deficiency and improvement of patients’ health status, asthenic component is preserved.
Perm Medical Journal. 2015;32(3):56-62
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CLINICOLABORATORY diagnostic CRITERIA FOR CHRONIC CYTOMEGALOVIRUS INFECTION IN EARLY AGE CHILDREN
Legotina N.S., Lvova I.I., Deryusheva A.V.
Abstract
Aim. To determine the clinicolaboratory diagnostic criteria for chronic cytomegalovirus infection among children of early age, so as to optimize diagnosis in conditions of children’s polyclinic. Materials and methods. The object of the study included immunocompromised children with verified cytomegalovirus infection (CMVI) using the method of qualitative saliva and urinary PCR by means of the test-system produced by “Central Scientific Research Institute of Epidemiology”, “AmpliSens CMV-screen/monitor-FL” (Moscow) (61 patients, total sampling). Patients aged 3-6 months (13 %), 6-12 months (21 %), 1-3 years (43 %), 3-7 years (23 %) were sent from Perm polyclinics to receive consultations, clarify the diagnosis and correct the therapy. Results. According to statistical reports of Perm children’s polyclinics for 2013, frequency of CMVI diagnosis in the group of early age children who frequently fall ill (23,1 %) was stated that makes half of cases of the other intrauterine infections (44,7 %). The group of immunocompromised ambulatory patients (61 children aged 3 months to 7 years) with verified chronic CMVI was examined. Clinicolaboratory secondary immunodeficiency (SID) markers from birth or the first months of life were revealed. A marked dysbiotic status of oropharynx associated with Epstein-Barr infection and type 6 herpesviral infection was diagnosed in 54,1 % of cases. Conclusions. Immunopathological syndromes were presented as infectious (77 %), lymphoproliferative (54,1 %) and allergic (44,3 %).
Perm Medical Journal. 2015;32(3):63-69
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COMPARATIVE CHARACTERISTIC OF DIFFERENT IMMUNOMODULATOR INTRODUCTION WAYS FOR PREVENTION OF PYOSEPTIC PANCREONECROTIC COMPLICATIONS
Tretyakov E.V.
Abstract
Aim. To prove the efficiency of using immunomodulator “Derinat” for patients with aseptic pancreatonecrosis and to compare the efficiency of rectal and intramuscular ways of drug introduction. Materials and methods. The group of comparison consisting of 30 patients diagnosed aseptic pancreatonecrosis received standard therapy; 30 patients of subgroup I of the main group received standard therapy and rectal introduction of immunomodulator; 30 patients of subgroup II received standard therapy and intramuscular introduction of immunomodulator. Immunomodulator “Derinat” raised the percentage ratio of T-lymphocytes, T-helpers, B-lymphocytes, phagocytes in patients of the main group versus the comparison group. Results. The lactulose/mannitol test indices in patients of subgroup I were significantly lower than those in subgroup II and the comparison group. Intramuscular and rectal use of immunomodulator “Derinat” reduced the number of complications: 53,3 ± 5,6 % in subgroup I and 62,7 ± 6,7 % in subgroup II versus the comparison group - 64,0 % ± 17,4 %. The number of operative interventions decreased: 50,0 ± 8,9 % and 49,9 ± 5,4 % in patients of subgroups I and II, respectively, versus the comparison group. Lethality was 13,3 ± 3,5 % and 16,7 ± 4,2 % in patients of subgroups I and II, respectively, in comparison group - 20,0 ± 3,4 %. Conclusions. Application of immunomodulator “Derinat” for prevention of pyoseptic complications is confirmed; maximum effect is reached with rectal introduction of the drug.
Perm Medical Journal. 2015;32(3):70-76
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DYNAMICS OF CLINICOFUNCTIONAL INDICES IN PATIENTS WITH CEREBRAL SPASTIC INFANTILE PARALYSIS AGAINST THE BACKGROUND OF COMPLEX THERAPY USING FUNCTIONAL ELECTROSTIMULATION IN SANATORIUM CONDITIONS
Radchenko N.V.
Abstract
Aim. To estimate the dynamics of clinicofunctional indices in patients with cerebral spastic infantile paralysis (CSIP) in complex structure of rehabilitation using functional electrostimulation by means of the apparatus “Myoneuroton”. Materials and methods. The efficiency of rehabilitation therapy conducted in 150 patients (aged 6 to 18 years), who were diagnosed CSIP in the form of spastic diplegia, was studied. Patients were divided into 2 groups: the main group ( n = 76) received complex rehabilitation including exercise therapy, massage, paraffin-ozokerit applications, needle reflexotherapy (NRT), functional electrostimulation with the apparatus “Myoneuroton”, the comparison group ( n = 74) - electrostimulation with the apparatus “Amplipulse-5”. Patients’ mean age was 12,5 ± 0,8 years. Results. After treatment, clinical studies carried out in the main group indicated the following: increase in force in mm. quadriceps femoris dex. et sin by 13 %, in mm. tibialis anterior dex. et sin. by 28 %; 8 % growth in range of motions in the knee and ankle joints, 26 % rise in the number of steps; 45 % increase in the mean pace; 96 % growth in duration of the right leg balance holding, 93 % - of the left leg. Conclusions. Electrostimulation significantly reduces manifestations of neurological deficit; stato-locomotor functions are improved versus the generally accepted methods of treatment.
Perm Medical Journal. 2015;32(3):77-81
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PECULIARITIES OF DISTRIBUTION OF MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS IN CHILDREN OF DIFFERENT AGE WITH ATOPIC DERMATITIS
Illek Y.Y., Zaitseva G.A., Suslova E.Y., Galanina A.V., Tarasova E.Y., Leushina N.P., Mischenko I.Y.
Abstract
Aim. To study the peculiar features of distribution of HLA-complex antigens in children of early age, preschool, junior school age and adolescents suffering from atopic dermatitis. Materials and methods. The study involved 128 children aged 8 months to 15 years suffering from disseminated moderately severe and severe atopic dermatitis, who were divided into 3 age groups and examined. Group I ( n = 76) included children of early age (patients’ age 8 months to 3 years), group 2 ( n = 32) - children of preschool and junior school age (patients’ age 5-10 years). group 3 ( n = 20) - adolescents (patients’ age 12-15 years). In patients with atopic dermatitis, serological typing by HLA antigens of class I and molecular typing of HLA-genes - class II HLA antigens was performed. Results. In children of early age, preschool, junior school age and adolescents, suffering from atopic dermatitis, associative relation with different major histocompatibility complex antigens was revealed that permitted to single out immunogenetic markers of this disease for patients of different age groups.
Perm Medical Journal. 2015;32(3):82-85
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PECULIARITIES OF PNEUMOCOCCAL PNEUMONIA COURSE IN MEDICAL PERSONNEL
Nikolenko V.V.
Abstract
Аim. To detect the clinical peculiarities of pneumococcal pneumonia course among medical personnel. Materials and methods. Over the period of 2001 to 2014, 49 patients diagnosed community-acquired pneumococcal pneumonia were examined on the basis of Perm Regional Clinical Infectious Hospital. Results. Analysis of the clinical course of S. pneumonia -induced community-acquired pneumococcal pneumonia in the group of medical personnel permitted to establish a number of peculiarities, which should be taken into account for diagnosis of these diseases. The disease was characterized by moderate and severe course with extensive pulmonary-tissue damage, clear complains and late response to bacterial inflammation in blood tests. Frequency of antibiotic-resistant S. pneumonia strains causing pulmonary-tissue damage was 16,3 % that exceeds the analogous index in the group of patients who do not belong to medical personnel. Conclusions. Peculiar features including difficulties in diagnosis of S. pneumonia -induced community-acquired pneumococcal pneumonia, severe course, significant number of cephalosporin and fluoroquinolone-resistant pneumococcal strains prove the need for specific prevention of medical personnel from pneumococcal infection.
Perm Medical Journal. 2015;32(3):86-92
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TOBACCO SMOKING FREQUENCY AMONG PREGNANT WOMEN AND ITS ASSOCIATION WITH OBSTETRIC COMPLICATIONS
Olina A.A., Meteleva T.A.
Abstract
Aim. To study the peculiar features of pregnancy course in smokers and to estimate the adequacy of preventive measures in high risk group for obstetric complications. Materials and methods. Health cards of 2270 pregnant women, who visited the Center of Family Planning and Prenatal Diagnosis of Perm State Medical University named after E. A. Wagner, were analyzed. Results. It was revealed that before pregnancy the share of smokers was 24,3 %. Among them, 78,5 % of women continued smoking during pregnancy, that is, prevalence of smoking among pregnant women is 190 per 1000. Hyperhomocysteinemia was detected in 69,5 % of smoking women. Conclusions. Every tenth pregnant woman is a smoker that permits to attribute her to the risk group for complicated pregnancy course from the early terms of pregnancy; smoking in most women is the cause of hyperhomocysteinemia and the risk factor for miscarriage and fetal growth retardation syndrome.
Perm Medical Journal. 2015;32(3):93-98
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QUALITY OF LIFE AS CRITERION FOR REHABILITATION EFFICIENCY IN CHILDREN WITH ATTENTION DEFICIT AND HYPERACTIVITY SYMPTOMS
Startsev A.A.
Abstract
Aim. To assess the dynamics of the quality of life in children with attention deficit and hyperactivity symptoms (ADHS) against the background of complex rehabilitation including hypoxihypercapnic training with respiratory simulator “Carbonic”. Materials and methods. Sixty three children (aged 10-15 years) diagnosed ADHS were divided into 2 randomized groups: the main group - 33 children receiving the basic rehabilitation complex with addition of hypoxihypercapnic training by means of respiratory training simulator “Carbonic”, the comparison group - 30 children receiving the basic rehabilitation complex alone. The quality of life was assessed by means of PedsQL 4.0 questionnaire before and after treatment. Results. Before treatment, the lowest quality of life was noted by the scales “Emotional State” and “Communication”. After treatment, there was statistically significant increase in the values by the scales “Emotional State” (by 43,1 % ( p < 0,05) in main group and by 20 % ( p < 0,05) in comparison group), “Communication” (by 67,2 % ( p < 0,05) in main group and by 31,8 % ( p < 0,05) in comparison group), “School” (by 17 % ( p < 0,05) in main group and by 12,9 % ( p < 0,05) in comparison group). Conclusions. After the course of rehabilitation including hypoxihypercapnic training with respiratory training simulator “Carbonic”, a reliable positive dynamics in the quality of life indices such as “Emotional State”, “Communication”, “School” was fixed. In the comparison group, there was also noted a reliable dynamics of these indices, but it is less significant than in the main group.
Perm Medical Journal. 2015;32(3):99-103
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REGIONAL PECULIARITIES OF ANTHROPOMETRIC CHARACTERISTICS IN CHILDREN OF SAMARA REGION
Burakova E.N.
Abstract
Aim. To analyze the peculiar features of anthropometric characteristics in children of Samara region. Materials and methods. During the period from 2007 to 2014 we examined 580 children aged 0-18 years. While studying, 4060 measurements were taken. To process the data the most adequate methods of mathematical statistics were used. Results. Reliable age changes in circumference and length sizes depending on sex were stated. The developed database permits to implement not only individual, but population estimation of the physical growth and development and can serve as a basis for creating software for anthropometric approach in clinics. Introduction of evaluation tables at children’s institutions, determination of risk groups for physical development disorders in children aged 0-18 years, who live in Samara, contributes to early diagnosis of developmental defects and timely administration of correcting measures. Conclusions. Taking into account the conducted studies, we can say that the periods of rapid growth are more marked in boys than in girls.
Perm Medical Journal. 2015;32(3):104-107
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CARNITINE CHLORIDE EFFECT ON FUNCTIONAL STATE OF MITOCHONDRIA IN CONDITIONS OF NITRIC OXIDE SYNTHESIS DEFICIT
Zvyagina V.I., Uryasiev O.M., Belskikh E.S., Medvedev D.V.
Abstract
Aim. To study the effect of carnitine chloride on functional state of mitochondria and endogenous carnitine content in conditions of L-NAME-induced decrease in nitric oxide synthesis (II). Materials and methods. Twenty four Wistar rats were divided into the following groups: the control group, the group with intraperitoneal introduction of L-NAME in the dose of 25 mg/kg for 7 days and the group receiving L-NAME by the analogous scheme against the background of introduction of carnitine chloride in the dose of 300 mg/kg for 21 days. The animals experienced the study of lactate dehydrogenase (LDG), succinate dehydrogenase (SDG), superoxide dismutase (SOD), lactate, nitric oxide (II), carnitine (total, free, bound) and nonetherified fatty acid concentrations in the rat cardiomyocyte mitochondria. Results. L-NAME in the dose of 25 mg/kg led to decrease in NO metabolite (by 21,5 %, p < 0,05) and lactate (by 56 %, p < 0,05) concentrations in myocardiocyte mitochondria; at the same time, it increased nonetherified fatty acid (by 290 %, p < 0,05) and total carnitine (by 162 %, p < 0,05) content as well as activity of all the three measured oxidoreductases in mitochondrial cardiac tissues as compared to the control animal indices (LDG by 160 %, p < 0,05; SDG by 109 %, p < 0,05; SOD by 133 %, p < 0,05). Preliminary introduction of carnitine chloride induced reliable significant growth of SDG (by 163 %, p < 0,05) and SOD (by 376 %, p < 0,05) activity, rise in NO metabolite (by 437 %, p < 0,05) and lactate (by 62 %, p < 0,05) concentrations, as well as to fall in fatty acid (37 %, p < 0,05) and total carnitine (by 35 %, p < 0,05) concentrations and LDG activity (by 50 %, p < 0,05). Conclusions. Preliminary administration of cortinine chloride in the dose of 300 mg/kg before introduction of L-NAME in the dose of 25 mg/kg normalizes carnitine homeostasis, prevents decrease in NO metabolite concentration, declines nonetherified fatty acid concentration and significantly elevates SOD and SDG activity in the rat cardiomyocyte mitochondria.
Perm Medical Journal. 2015;32(3):108-114
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MORPHOMETRIC ANALYSIS OF ESOPHAGEAL CIRCULATORY INTRAORGAN COMPONENT DIAMETERS IN HUMAN FETUSES AND ADULTS
Gelashvili P.A., Vodolazov A.V., Tokarev V.N.
Abstract
Aim. To carry out morphological analysis of the changes in diameter of fetal and adult intraorgan microvessels of the lower third of esophagus. Materials and methods. Anatomical and histological methods were used to reveal the components of hemomicrocirculatory canal. The lower third of esophagus was studied in 18 human fetuses in the terms of 13, 23, 26, 32, 36 weeks of development, in 7 dead adults aged 41 to 50 years, who did not suffer from esophageal and hepatic diseases in the course of life. Results. Reliable age changes in external diameter of human esophageal arteries, veins, arterioles, capillaries, postcapillaries and venules using nonparametric statistical methods were detected. Conclusions. In dynamics of prenatal development, there occur unequal changes in capillary diameter: increase during the week 23 of gestation (up to 4,98 ± 0,11 mcm, p = 0,005), decrease beginning from the weeks 26 to 32 (4,07 ± 0,14 mcm, p = 0,000) and rise by the week 36 (5,40 ± 0,17 mcm, p = 0,000). Diameter of capillaries of the lower third of esophagus in adults is 9,43 ± 0,24 mcm ( p = 0,000).
Perm Medical Journal. 2015;32(3):115-119
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EXPERIMENTAL NSAID-INDUCED HEPATOPATHY CAUSED BY LONG USE OF NIMESULIDE
Lazarenko L.V., Kosareva P.V., Samodelkin E.I., Khorinko V.P.
Abstract
Aim. To assess the character of hepatic tissue lesion in experimental animals when modeling NSAID-hepatopathy with generally accepted histological methods. Materials and methods. Modeling of nonsteroid antiinflammatory drug-induced hepatic lesion in nonlinear white rats was performed with peroral introduction of nimesulide (Nise®) during 21 days in the following doses: 0,5 (therapeutic dose); 2,5 and 5 mg/kg. The control group was used for comparison. By the end of this experiment, histological material (hepatic tissue samples) was taken with euthanasia rules observed. Histological methods were used by standard protocols. Results. Peroral introduction of nimesulid during 21 days caused pathomorphological changes in hepatic tissue of experimental animals, typical for the toxic damage of the organ: sinusoid capillary dilatation, dystrophy and necrosis foci; at the same time, dose-dependent effect was revealed. Lymphocytic infiltrates were detected in hepatic tissue of animals perorally introduced nimesulide during 21 days; frequency of their detection increased with growth of the drug dose; the revealed changes require further study and explanation.
Perm Medical Journal. 2015;32(3):120-123
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PULSE VISUALIZATION WITH VIDEOSIGNAL
Chechulin V.L., Brokhin L.Y., Cherepanova Y.A., Anisimov P.I., Lyakh E.L.
Abstract
Materials and methods. The following principles of noisy signal filtration were used: linear smoothing forming the basic signal values, averaging by the period equal to mean pulse period, linear smoothing filtrating difference in videosignal and the obtained basic signal. The obtained difference is multiplied by the gain constant and added to the initial or the obtained basic signal. Conclusions. It was indicated that dynamic videoamplification, which does not apply filtration by the period equal to the mean value of pulse period, is of little use owing to artifacts available in the form of changes in brightness of lighting or relocation of the examined person. The offered algorithm differs from foreign analogs using dynamic amplification. The described mathematical methods for videosignal amplification are tested on videosignal of human forehead imaging.
Perm Medical Journal. 2015;32(3):124-130
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GENERALIZED HEMOPHILIC INFECTION IN PREMATURE IMMUNOCOMPROMISED INFANT OF THE FIRST YEAR OF LIFE
Grishkina L.Y., Lvova I.I., Legotina N.S., Batrakova G.V., Gireva T.V., Trefilov I.N., Ogorodova N.I., Melnikova A.V., Dovganyuk M.V.
Abstract
Generalized hemophilic infection in premature 7-month-old immunocompromised infant, which required specialized multitype hospital treatment, is described in the paper.
Perm Medical Journal. 2015;32(3):131-135
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SEVERE VISCERAL LEISHMANIASIS IN A CHILD OF EARLY AGE AGAINST THE BACKGROUND OF OPPORTUNISTIC HERPESVIRUS INFECTIONS
Lvova I.I., Legotina N.S., Deryusheva A.V., Batrakova G.V., Gireva T.V., Banko N.V., Uvarova E.Y., Ramazanova E.F., Lazukov E.P.
Abstract
A clinical case of severe visceral leishmaniasis in a child aged 3 years and 2 months developing against the background of activation of mixed intrauterine herpesvirus infection (cytomegalovirus infection and Epstein-Barr virus infection) is described in the paper.
Perm Medical Journal. 2015;32(3):136-140
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